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Absorbance at and nm was also measured to determine nucleic acid purity. Since the discovery that inheritance of the apoE4 allele increases risk and decreases the age of onset of late-onset AD 2the putative roles of apoE in AD pathogenesis have been a topic of intensive focus.

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Taken together, the relative amyloid burden across murine and human apoE is: Close the windows and doors and let sit overnight. Whiteway NL, St.

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CSF was isolated from the cisterna magna as described Reconstitution of human apoE isoforms into AD mice greatly delays amyloid deposition relative to murine apoE, but retains the human isoform-specific influence on amyloid accumulation 12 — An additional two sections per mouse were first stained with resorufin, which selectively binds vascular amyloid If necessary, repeat until the stain is gone.

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The substrate and product of the LCAT reaction were separated by thin-layer chromatography using flexible silica polyester backed plates Whatman, Protein levels were normalized to GAPDH or albumin and expressed as fold difference compared with controls.

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